A Rapid, Equipment-Free Method for Detecting Phytophthora infestans in the Field Using a Lateral Flow Strip-Based Recombinase Polymerase Amplification Assay.
Identifieur interne : 000322 ( Main/Exploration ); précédent : 000321; suivant : 000323A Rapid, Equipment-Free Method for Detecting Phytophthora infestans in the Field Using a Lateral Flow Strip-Based Recombinase Polymerase Amplification Assay.
Auteurs : Xinyu Lu [République populaire de Chine] ; Ying Zheng [République populaire de Chine] ; Fan Zhang [République populaire de Chine] ; Jia Yu [République populaire de Chine] ; Tingting Dai [République populaire de Chine] ; Rongbo Wang [République populaire de Chine] ; Yuee Tian [République populaire de Chine] ; Heng Xu [République populaire de Chine] ; Danyu Shen [République populaire de Chine] ; Daolong Dou [République populaire de Chine]Source :
- Plant disease [ 0191-2917 ] ; 2020.
Descripteurs français
- KwdFr :
- MESH :
- Wicri :
- geographic : République populaire de Chine.
English descriptors
- KwdEn :
- MESH :
- chemical , genetics : Recombinases.
- chemical : DNA Primers.
- geographic : China.
- genetics : Phytophthora infestans.
- Nucleic Acid Amplification Techniques.
Abstract
Late blight, caused by the oomycete Phytophthora infestans, is a major constraint on the production of potatoes and tomatoes as well as a constant threat to global food security. An early diagnostic tool is important for the effective management of late blight in the field. Here, in combination with a simplified DNA extraction method, we developed a lateral flow strip-based recombinase polymerase amplification (LF-RPA) assay for the rapid, equipment-free detection of P. infestans. This assay targets the Ras-related protein (Ypt1) gene and can be performed over a wide range of temperatures (25 to 45°C). All 12 P. infestans isolates yielded positive detection results using the LF-RPA assay, and no cross-reaction occurred with related oomycetes or fungal species. With this assay, the detection limit was 500 fg of genomic DNA in optimized conditions. Furthermore, by combining a simplified polyethylene glycol-NaOH method for extracting DNA from plant samples, the entire LF-RPA assay enabled the detection of P. infestans within 30 min with no specialized equipment. When applied to field samples, it successfully detected P. infestans in naturally diseased potato plants from eight different fields in China. Therefore, the LF-RPA assay is simple, rapid, and cost-effective and has potential for further development as a kit for diagnosing late blight in resource-limited settings or even on-site.
DOI: 10.1094/PDIS-01-20-0203-SC
PubMed: 32924873
Affiliations:
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Fuzhou 350013, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Institute of Plant Protection, Fujian Academy of Agricultural Sciences, Fuzhou 350013</wicri:regionArea><placeName><settlement type="city">Fuzhou</settlement><region type="province">Fujian</region></placeName></affiliation></author><author><name sortKey="Tian, Yuee" sort="Tian, Yuee" uniqKey="Tian Y" first="Yuee" last="Tian">Yuee Tian</name><affiliation wicri:level="1"><nlm:affiliation>Department of Plant Protection, Henan University of Science and Technology, Luoyang 471000, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Department of Plant Protection, Henan University of Science and Technology, Luoyang 471000</wicri:regionArea><wicri:noRegion>Luoyang 471000</wicri:noRegion></affiliation></author><author><name sortKey="Xu, Heng" sort="Xu, Heng" uniqKey="Xu H" first="Heng" last="Xu">Heng Xu</name><affiliation wicri:level="1"><nlm:affiliation>College of Plant Protection, Nanjing Agricultural University, Nanjing 210095, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>College of Plant Protection, Nanjing Agricultural University, Nanjing 210095</wicri:regionArea><wicri:noRegion>Nanjing 210095</wicri:noRegion></affiliation></author><author><name sortKey="Shen, Danyu" sort="Shen, Danyu" uniqKey="Shen D" first="Danyu" last="Shen">Danyu Shen</name><affiliation wicri:level="1"><nlm:affiliation>College of Plant Protection, Nanjing Agricultural University, Nanjing 210095, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>College of Plant Protection, Nanjing Agricultural University, Nanjing 210095</wicri:regionArea><wicri:noRegion>Nanjing 210095</wicri:noRegion></affiliation></author><author><name sortKey="Dou, Daolong" sort="Dou, Daolong" uniqKey="Dou D" first="Daolong" last="Dou">Daolong Dou</name><affiliation wicri:level="1"><nlm:affiliation>College of Plant Protection, Nanjing Agricultural University, Nanjing 210095, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>College of Plant Protection, Nanjing Agricultural University, Nanjing 210095</wicri:regionArea><wicri:noRegion>Nanjing 210095</wicri:noRegion></affiliation></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="2020">2020</date><idno type="RBID">pubmed:32924873</idno><idno type="pmid">32924873</idno><idno type="doi">10.1094/PDIS-01-20-0203-SC</idno><idno type="wicri:Area/Main/Corpus">000061</idno><idno type="wicri:explorRef" wicri:stream="Main" wicri:step="Corpus" wicri:corpus="PubMed">000061</idno><idno type="wicri:Area/Main/Curation">000061</idno><idno type="wicri:explorRef" wicri:stream="Main" wicri:step="Curation">000061</idno><idno type="wicri:Area/Main/Exploration">000061</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en">A Rapid, Equipment-Free Method for Detecting <i>Phytophthora infestans</i> in the Field Using a Lateral Flow Strip-Based Recombinase Polymerase Amplification Assay.</title><author><name sortKey="Lu, Xinyu" sort="Lu, Xinyu" uniqKey="Lu X" first="Xinyu" last="Lu">Xinyu Lu</name><affiliation wicri:level="1"><nlm:affiliation>College of Plant Protection, Nanjing Agricultural University, Nanjing 210095, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>College of Plant Protection, Nanjing Agricultural University, Nanjing 210095</wicri:regionArea><wicri:noRegion>Nanjing 210095</wicri:noRegion></affiliation></author><author><name sortKey="Zheng, Ying" sort="Zheng, Ying" uniqKey="Zheng Y" first="Ying" last="Zheng">Ying Zheng</name><affiliation wicri:level="1"><nlm:affiliation>College of Plant Protection, Nanjing Agricultural University, Nanjing 210095, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>College of Plant Protection, Nanjing Agricultural University, Nanjing 210095</wicri:regionArea><wicri:noRegion>Nanjing 210095</wicri:noRegion></affiliation></author><author><name sortKey="Zhang, Fan" sort="Zhang, Fan" uniqKey="Zhang F" first="Fan" last="Zhang">Fan Zhang</name><affiliation wicri:level="1"><nlm:affiliation>College of Plant Protection, Nanjing Agricultural University, Nanjing 210095, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>College of Plant Protection, Nanjing Agricultural University, Nanjing 210095</wicri:regionArea><wicri:noRegion>Nanjing 210095</wicri:noRegion></affiliation></author><author><name sortKey="Yu, Jia" sort="Yu, Jia" uniqKey="Yu J" first="Jia" last="Yu">Jia Yu</name><affiliation wicri:level="1"><nlm:affiliation>College of Plant Protection, Nanjing Agricultural University, Nanjing 210095, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>College of Plant Protection, Nanjing Agricultural University, Nanjing 210095</wicri:regionArea><wicri:noRegion>Nanjing 210095</wicri:noRegion></affiliation></author><author><name sortKey="Dai, Tingting" sort="Dai, Tingting" uniqKey="Dai T" first="Tingting" last="Dai">Tingting Dai</name><affiliation wicri:level="1"><nlm:affiliation>College of Forestry, Nanjing Forestry University, Nanjing 210037, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>College of Forestry, Nanjing Forestry University, Nanjing 210037</wicri:regionArea><wicri:noRegion>Nanjing 210037</wicri:noRegion></affiliation></author><author><name sortKey="Wang, Rongbo" sort="Wang, Rongbo" uniqKey="Wang R" first="Rongbo" last="Wang">Rongbo Wang</name><affiliation wicri:level="1"><nlm:affiliation>Institute of Plant Protection, Fujian Academy of Agricultural Sciences, Fuzhou 350013, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Institute of Plant Protection, Fujian Academy of Agricultural Sciences, Fuzhou 350013</wicri:regionArea><placeName><settlement type="city">Fuzhou</settlement><region type="province">Fujian</region></placeName></affiliation></author><author><name sortKey="Tian, Yuee" sort="Tian, Yuee" uniqKey="Tian Y" first="Yuee" last="Tian">Yuee Tian</name><affiliation wicri:level="1"><nlm:affiliation>Department of Plant Protection, Henan University of Science and Technology, Luoyang 471000, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Department of Plant Protection, Henan University of Science and Technology, Luoyang 471000</wicri:regionArea><wicri:noRegion>Luoyang 471000</wicri:noRegion></affiliation></author><author><name sortKey="Xu, Heng" sort="Xu, Heng" uniqKey="Xu H" first="Heng" last="Xu">Heng Xu</name><affiliation wicri:level="1"><nlm:affiliation>College of Plant Protection, Nanjing Agricultural University, Nanjing 210095, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>College of Plant Protection, Nanjing Agricultural University, Nanjing 210095</wicri:regionArea><wicri:noRegion>Nanjing 210095</wicri:noRegion></affiliation></author><author><name sortKey="Shen, Danyu" sort="Shen, Danyu" uniqKey="Shen D" first="Danyu" last="Shen">Danyu Shen</name><affiliation wicri:level="1"><nlm:affiliation>College of Plant Protection, Nanjing Agricultural University, Nanjing 210095, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>College of Plant Protection, Nanjing Agricultural University, Nanjing 210095</wicri:regionArea><wicri:noRegion>Nanjing 210095</wicri:noRegion></affiliation></author><author><name sortKey="Dou, Daolong" sort="Dou, Daolong" uniqKey="Dou D" first="Daolong" last="Dou">Daolong Dou</name><affiliation wicri:level="1"><nlm:affiliation>College of Plant Protection, Nanjing Agricultural University, Nanjing 210095, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>College of Plant Protection, Nanjing Agricultural University, Nanjing 210095</wicri:regionArea><wicri:noRegion>Nanjing 210095</wicri:noRegion></affiliation></author></analytic><series><title level="j">Plant disease</title><idno type="ISSN">0191-2917</idno><imprint><date when="2020" type="published">2020</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>China (MeSH)</term><term>DNA Primers (MeSH)</term><term>Nucleic Acid Amplification Techniques (MeSH)</term><term>Phytophthora infestans (genetics)</term><term>Recombinases (genetics)</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>Amorces ADN (MeSH)</term><term>Chine (MeSH)</term><term>Phytophthora infestans (génétique)</term><term>Recombinases (génétique)</term><term>Techniques d'amplification d'acides nucléiques (MeSH)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="genetics" xml:lang="en"><term>Recombinases</term></keywords><keywords scheme="MESH" type="chemical" xml:lang="en"><term>DNA Primers</term></keywords><keywords scheme="MESH" type="geographic" xml:lang="en"><term>China</term></keywords><keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>Phytophthora infestans</term></keywords><keywords scheme="MESH" qualifier="génétique" xml:lang="fr"><term>Phytophthora infestans</term><term>Recombinases</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Nucleic Acid Amplification Techniques</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Amorces ADN</term><term>Chine</term><term>Techniques d'amplification d'acides nucléiques</term></keywords><keywords scheme="Wicri" type="geographic" xml:lang="fr"><term>République populaire de Chine</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Late blight, caused by the oomycete <i>Phytophthora infestans</i>, is a major constraint on the production of potatoes and tomatoes as well as a constant threat to global food security. An early diagnostic tool is important for the effective management of late blight in the field. Here, in combination with a simplified DNA extraction method, we developed a lateral flow strip-based recombinase polymerase amplification (LF-RPA) assay for the rapid, equipment-free detection of <i>P</i>. <i>infestans</i>. This assay targets the Ras-related protein (<i>Ypt1</i>) gene and can be performed over a wide range of temperatures (25 to 45°C). All 12 <i>P</i>. <i>infestans</i> isolates yielded positive detection results using the LF-RPA assay, and no cross-reaction occurred with related oomycetes or fungal species. With this assay, the detection limit was 500 fg of genomic DNA in optimized conditions. Furthermore, by combining a simplified polyethylene glycol-NaOH method for extracting DNA from plant samples, the entire LF-RPA assay enabled the detection of <i>P. infestans</i> within 30 min with no specialized equipment. When applied to field samples, it successfully detected <i>P</i>. <i>infestans</i> in naturally diseased potato plants from eight different fields in China. Therefore, the LF-RPA assay is simple, rapid, and cost-effective and has potential for further development as a kit for diagnosing late blight in resource-limited settings or even on-site.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" IndexingMethod="Automated" Owner="NLM"><PMID Version="1">32924873</PMID><DateCompleted><Year>2020</Year><Month>11</Month><Day>03</Day></DateCompleted><DateRevised><Year>2020</Year><Month>11</Month><Day>03</Day></DateRevised><Article PubModel="Print-Electronic"><Journal><ISSN IssnType="Print">0191-2917</ISSN><JournalIssue CitedMedium="Print"><Volume>104</Volume><Issue>11</Issue><PubDate><Year>2020</Year><Month>Nov</Month></PubDate></JournalIssue><Title>Plant disease</Title><ISOAbbreviation>Plant Dis</ISOAbbreviation></Journal><ArticleTitle>A Rapid, Equipment-Free Method for Detecting <i>Phytophthora infestans</i> in the Field Using a Lateral Flow Strip-Based Recombinase Polymerase Amplification Assay.</ArticleTitle><Pagination><MedlinePgn>2774-2778</MedlinePgn></Pagination><ELocationID EIdType="doi" ValidYN="Y">10.1094/PDIS-01-20-0203-SC</ELocationID><Abstract><AbstractText>Late blight, caused by the oomycete <i>Phytophthora infestans</i>, is a major constraint on the production of potatoes and tomatoes as well as a constant threat to global food security. An early diagnostic tool is important for the effective management of late blight in the field. Here, in combination with a simplified DNA extraction method, we developed a lateral flow strip-based recombinase polymerase amplification (LF-RPA) assay for the rapid, equipment-free detection of <i>P</i>. <i>infestans</i>. This assay targets the Ras-related protein (<i>Ypt1</i>) gene and can be performed over a wide range of temperatures (25 to 45°C). All 12 <i>P</i>. <i>infestans</i> isolates yielded positive detection results using the LF-RPA assay, and no cross-reaction occurred with related oomycetes or fungal species. With this assay, the detection limit was 500 fg of genomic DNA in optimized conditions. Furthermore, by combining a simplified polyethylene glycol-NaOH method for extracting DNA from plant samples, the entire LF-RPA assay enabled the detection of <i>P. infestans</i> within 30 min with no specialized equipment. When applied to field samples, it successfully detected <i>P</i>. <i>infestans</i> in naturally diseased potato plants from eight different fields in China. Therefore, the LF-RPA assay is simple, rapid, and cost-effective and has potential for further development as a kit for diagnosing late blight in resource-limited settings or even on-site.</AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Lu</LastName><ForeName>Xinyu</ForeName><Initials>X</Initials><AffiliationInfo><Affiliation>College of Plant Protection, Nanjing Agricultural University, Nanjing 210095, China.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Zheng</LastName><ForeName>Ying</ForeName><Initials>Y</Initials><AffiliationInfo><Affiliation>College of Plant Protection, Nanjing Agricultural University, Nanjing 210095, China.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Zhang</LastName><ForeName>Fan</ForeName><Initials>F</Initials><AffiliationInfo><Affiliation>College of Plant Protection, Nanjing Agricultural University, Nanjing 210095, China.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Yu</LastName><ForeName>Jia</ForeName><Initials>J</Initials><AffiliationInfo><Affiliation>College of Plant Protection, Nanjing Agricultural University, Nanjing 210095, China.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Dai</LastName><ForeName>Tingting</ForeName><Initials>T</Initials><Identifier Source="ORCID">http://orcid.org/0000-0003-0534-6724</Identifier><AffiliationInfo><Affiliation>College of Forestry, Nanjing Forestry University, Nanjing 210037, China.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Wang</LastName><ForeName>Rongbo</ForeName><Initials>R</Initials><AffiliationInfo><Affiliation>Institute of Plant Protection, Fujian Academy of Agricultural Sciences, Fuzhou 350013, China.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Tian</LastName><ForeName>Yuee</ForeName><Initials>Y</Initials><AffiliationInfo><Affiliation>Department of Plant Protection, Henan University of Science and Technology, Luoyang 471000, China.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Xu</LastName><ForeName>Heng</ForeName><Initials>H</Initials><AffiliationInfo><Affiliation>College of Plant Protection, Nanjing Agricultural University, Nanjing 210095, China.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Shen</LastName><ForeName>Danyu</ForeName><Initials>D</Initials><Identifier Source="ORCID">http://orcid.org/0000-0002-2973-0010</Identifier><AffiliationInfo><Affiliation>College of Plant Protection, Nanjing Agricultural University, Nanjing 210095, China.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Dou</LastName><ForeName>Daolong</ForeName><Initials>D</Initials><AffiliationInfo><Affiliation>College of Plant Protection, Nanjing Agricultural University, Nanjing 210095, China.</Affiliation></AffiliationInfo></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType></PublicationTypeList><ArticleDate DateType="Electronic"><Year>2020</Year><Month>09</Month><Day>14</Day></ArticleDate></Article><MedlineJournalInfo><Country>United States</Country><MedlineTA>Plant Dis</MedlineTA><NlmUniqueID>9882809</NlmUniqueID><ISSNLinking>0191-2917</ISSNLinking></MedlineJournalInfo><ChemicalList><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D017931">DNA Primers</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D045522">Recombinases</NameOfSubstance></Chemical></ChemicalList><CitationSubset>IM</CitationSubset><MeshHeadingList><MeshHeading><DescriptorName UI="D002681" MajorTopicYN="N" Type="Geographic">China</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D017931" MajorTopicYN="N">DNA Primers</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D021141" MajorTopicYN="N">Nucleic Acid Amplification Techniques</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D055750" MajorTopicYN="Y">Phytophthora infestans</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D045522" MajorTopicYN="N">Recombinases</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName></MeshHeading></MeshHeadingList><KeywordList Owner="NOTNLM"><Keyword MajorTopicYN="N">RPA</Keyword><Keyword MajorTopicYN="N">diagnostics</Keyword><Keyword MajorTopicYN="N">isothermal amplification</Keyword><Keyword MajorTopicYN="N">oomycetes</Keyword><Keyword MajorTopicYN="N">visualization</Keyword></KeywordList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="pubmed"><Year>2020</Year><Month>9</Month><Day>15</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2020</Year><Month>11</Month><Day>4</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="entrez"><Year>2020</Year><Month>9</Month><Day>14</Day><Hour>15</Hour><Minute>40</Minute></PubMedPubDate></History><PublicationStatus>ppublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">32924873</ArticleId><ArticleId IdType="doi">10.1094/PDIS-01-20-0203-SC</ArticleId></ArticleIdList></PubmedData></pubmed><affiliations><list><country><li>République populaire de Chine</li></country><region><li>Fujian</li></region><settlement><li>Fuzhou</li></settlement></list><tree><country name="République populaire de Chine"><noRegion><name sortKey="Lu, Xinyu" sort="Lu, Xinyu" uniqKey="Lu X" first="Xinyu" last="Lu">Xinyu Lu</name></noRegion><name sortKey="Dai, Tingting" sort="Dai, Tingting" uniqKey="Dai T" first="Tingting" last="Dai">Tingting Dai</name><name sortKey="Dou, Daolong" sort="Dou, Daolong" uniqKey="Dou D" first="Daolong" last="Dou">Daolong Dou</name><name sortKey="Shen, Danyu" sort="Shen, Danyu" uniqKey="Shen D" first="Danyu" last="Shen">Danyu Shen</name><name sortKey="Tian, Yuee" sort="Tian, Yuee" uniqKey="Tian Y" first="Yuee" last="Tian">Yuee Tian</name><name sortKey="Wang, Rongbo" sort="Wang, Rongbo" uniqKey="Wang R" first="Rongbo" last="Wang">Rongbo Wang</name><name sortKey="Xu, Heng" sort="Xu, Heng" uniqKey="Xu H" first="Heng" last="Xu">Heng Xu</name><name sortKey="Yu, Jia" sort="Yu, Jia" uniqKey="Yu J" first="Jia" last="Yu">Jia Yu</name><name sortKey="Zhang, Fan" sort="Zhang, Fan" uniqKey="Zhang F" first="Fan" last="Zhang">Fan Zhang</name><name sortKey="Zheng, Ying" sort="Zheng, Ying" uniqKey="Zheng Y" first="Ying" last="Zheng">Ying Zheng</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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HfdIndexSelect -h $EXPLOR_AREA/Data/Main/Exploration/RBID.i -Sk "pubmed:32924873" \
| HfdSelect -Kh $EXPLOR_AREA/Data/Main/Exploration/biblio.hfd \
| NlmPubMed2Wicri -a PhytophthoraV1
| This area was generated with Dilib version V0.6.38. |  |